• 姓名: 马慧敏
  • 性别: 女
  • 职务: 
  • 职称: 副研究员
  • 学历: 博士研究生
  • 电话: 020-38350661
  • 传真: 020-8529-0706
  • 电子邮件: mahuimin@gig.ac.cn
  • 通讯地址: 广州市天河区科华街511号
    简  历:
  • 马慧敏,副研究员,博士,从事环境污染物的早期健康效应研究,研究了大气细颗粒物(包括PM2.5PM0.1等),环境中的重金属和有机物(苯,三氯生等)等对人体健康的影响,利用体内体外实验方法,筛选大气颗粒物中的有效毒性组分,筛选重金属人体健康影响的安全阈值,筛选环境有机污染物等对人体的特异性生物标志物,阐述对人体的毒作用模式。

    针对环境中重金属的污染问题,对广州地区一个重金属污染区的适龄儿童进行智力测试,结合儿童的血液和尿液中的四种重金属的水平数据,分析出四种重金属之间有显著的联合效应,其中血铅仍然是诱导儿童智力降低的主要因素,结合体外实验,筛选出2个印记基因DNA甲基化生物标志物。结果发现血铅在低于国家检测线100 μg/L 的情况下(约57 μg/L以下),也会和智力有显著的负相关性,提示了国家政府部门需要对血铅的相关安全标准再重新进行审视,结论发表在ES&TEP杂志上

    分析PM2.5样品各组分中的有效毒性物质,初步结果表明大气颗粒物中类腐殖酸类化合物(Huminc-like Substances, HULIS)可能是PM2.5中诱导炎症和凋亡的主要毒性组分,并随后开展了人群样品的HULIS毒性鉴定。利用细胞模型检测毒性,傅里叶变换离子回旋共振质谱仪分析化合物组成信息,加速器质谱等分析不同污染物的来源信息,核磁共振等技术探索有机物结构信息,制备性液相色谱分离不同化合物组分等。揭示了大气颗粒物中的HULIS组分可能是颗粒物水溶性组分中的诱导炎症效应和凋亡的主要组分之一,其中含氧基团,芳香环和双键结构等对炎症和凋亡的贡献较大,这些化合物可能与玉米秸秆等生物质燃烧、交通源排放和燃煤排放有关。开展了人群定组研究(panel study),采集了定组人群的样品,分析大气PM2.5/PM0.1暴露对人体心肺等功能损伤,颗粒物各组分对健康的危害及其贡献大小,各组分的毒作用机制,研究结果发表在ES&TEP等杂志上,并选为当期的内封面。

    在国内首次建立HPLC-MS/MS全基因组DNA甲基化和MALDI-TOF-MS/MS分析组蛋白修饰的检测方法,分析了环境中普遍存在的三氯生和苯及其代谢物低浓度暴露诱导的毒性效应,并筛选出了潜在的分子标志物。关于三氯生的文章,发表在毒理学专业的期刊Toxicology in vitro Chemosphere上,被英国知名的文献推荐平台F1000prime推荐为“非常好”的论文,并评价了论文结果对三氯生肝毒性的贡献。

     

    学习工作简历:

    1999-2003:安徽医科大学,生物技术专业, 学士学位;

    2003-2009:中国科学院广州地球化学研究所,博士学位;

    2007-2008Jacobs UniversityGerman,  Biochemistry, 联合培养博士生;

    2009 – 2011有机地球化学国家重点实验室, 中国科学院广州地球化学研究所,助理研究员 

    2011 至今有机地球化学国家重点实验室, 中国科学院广州地球化学研究所副研究员;

    2018 – 2019Lancaster Environmental Center, Lancaster University, Lancaster UK, Visiting Scholar.

    社会任职:
  • · 2019年被聘为 “中国环境诱变学会的副监事长;

    · 2017年被聘为中国毒理学会生化与分子毒理专业委员会委员.

    研究方向:
  • 环境表观遗传学

    获奖及荣誉:
    代表论著:
  • [1].Cong Wan, Shangxia Pan, Lifeng Lin, Jun Li, Guanghui Dong, Kevin C. Jones, Fei Liu, Daochuan Li, Jing Liu, Zhiqiang Yu, Gan Zhang, and Huimin Ma*, DNA Methylation Biomarkers of IQ Reduction are Associated with Long Term Lead Exposure in School Aged Children in Southern China . Environ Sci Technol, 2020, http://dx.doi.org/10.1021/acs.est.0c01696;

    [2].Yaohui Liang, Liwen Hu, Jun Li, Fei Liu, Kevin C. Jones, Daochuan Li, Jing Liu, Duohong Chen, Jing You, Zhiqiang Yu, Gan Zhang, Guanghui Dong, Huimin Ma*, Short-term personal PM2.5 exposure and change in DNA methylation of imprinted genes: Panel study of healthy young adults in Guangzhou city, China, Environmental Pollution, 2021 (accepted).

    [3].Huimin Ma, Jun Li, Cong Wan, Yaohui Liang, Yangzhi Mo, Xiangyun Zhang, Guanghui Dong, Liwen Hu, , Xiaowen Zeng, Kevin C. Jones, Zhiqiang Yu, Gan Zhang*Potential chemical components and signaling pathways that induce apoptosis in PM2.5 from 10 major cities in China, Environ Sci Technol. (under reviewed)

    [4].Huimin Ma, Jun Li, Cong Wan, Yaohui Liang, Xiangyun Zhang, Guanghui Dong, Liwen Hu, Boyi Yang, Xiaowen Zeng, Tao Su, Shaoyou Lu, Shejun Chen, Mahdi Safaei Khorram, Guoying Sheng, Xinming Wang, Bixian Mai, Zhiqiang Yu*, Gan Zhang* Inflammation response of water-soluble fractions in atmospheric fine particulates: a seasonal observation in 10 large Chinese cities Environ Sci Technol. 2019, 53(7):3782-3790.

    [5].Shangxia Pan, Lifeng Lin, Fan Zeng, Jianpeng Zhang, Guanghui Dong, Boyi Yang, You Jing, Shejun Chen, Gan Zhang, Zhiqiang Yu, Guoying Sheng, Huimin Ma*, Effects of lead, cadmium, arsenic, and mercury co-exposure on children's intelligence quotient in an industrialized area of southern China, Environmental Pollution, 2018, 20(235):47-54.

    [6].Liudan Zeng, Huimin Ma*, Shangxia Pan, Jing You, Gan Zhang, Zhiqing Yu,  Guoying Sheng, Jiamo FuLINE-1 gene hypomethylation and p16 gene hypermethylation in HepG2 cells induced by low-dose and long-term Triclosan exposure: The role of hydroxyl group, Toxicology in vitro, 201634:35-44.

    [7].Junjie Hu, Huimin Ma*, Wenbing Zhang*, Zhiqing Yu, Guoying Sheng, Jiamo FuEffects of benzene and its metabolites on global DNA methylation in human normal hepatic L02 cellsEnvironmental Toxicology. 2014, 29:108-116.

    [8].Huimin Ma*, Liujin Zheng, Yuhua Li, Shaxia Pan, Zhiqiang Yu, Gan Zhang, Guoying Sheng, Jiamo FuTriclosan Reduces the Levels of Global DNA Methylation in HepG2 CellsChemosphere, 2013,90:1023-1029.

    [9].Junjie Hu, Wenbing Zhang*, Huimin Ma*, Yunmei Cai, Guoying Sheng, Jiamo Fu.Simultaneous determination of 8-hydroxy-2 '-deoxyguanosine and 5-methyl-2 '-deoxycytidine in DNA sample by highperformance liquid chromatography/positive electrospray ionization tandem mass spectrometry. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2010, 878:2765-2769.

    [10].Huimin Ma, Wenbing Zhang*, Junjie Hu, Zhiqiang Yu, Yong Chen, Qiong luo, Xiaoliu Shi, Yingying Zhang, Renfang Song, Zhen Zhou, Guoying Sheng and Jiamo Fu, “Analysis of global DNA methylation level in human blood using high-performance liquid chromatography/tandem electrospray ionization mass spectrometry (HPLC-ESI-MS/MS)”, European Journal of Mass Spectrometry, 2009 ,15: 555-561.

    [11].Huimin Ma Wenbing Zhang* Renfang Song Huayong Chen Guoying Sheng Zhen Zhou and Jiamo Fu “Characterization of methylated and unmethlyated CpG oligodeoxynucleotides by electrospray ionization tandem mass spectrometry” European Journal of Mass Spectrometry200511581-589

    [12].Yang B, Qian Z, Vaughn M, Nelson E, Dharmage S, Heinrich J, Lin S, Lawrence WR, Ma H, Chen D, Hu L, Zeng X, Xu S, Zhang C, Dong G. Is prehypertension more strongly associated with long-term ambient air pollution exposure than hypertension? Findings from the 33 Communities Chinese Health Study. Environmental Pollution. 2017;229:696-704.

    [13].潘尚霞,马慧敏*,林立丰,张建鹏,万聪,环境铅暴露对儿童智力和精神运动功能影响研究,华南预防医学,20175412-417

    [14].曾柳丹,马慧敏,*, 张干, 于志强, 盛国英,傅家谟, BDE-208BDE-209诱导了LINE-1基因低甲基化和ESR1基因高甲基化,生态毒理学报,20162163-169

    [15].王晶晶、马慧敏*、李宝才*、郑刘进、潘尚霞、黎玉华、盛国英、傅家谟,苯代谢物氢醌对人体肝细胞内DNA甲基转移酶基因和蛋白表达的影响 ,昆明理工大学学报(自然科学版), 2013,38:90-95.

    [16].胡俊杰、马慧敏*、张文兵、李林朋、于志强、盛国英、傅家谟,苯急性暴露对Sprague-Dawley大鼠全基因组DNA 甲基化的影响,生态环境学报,2010, 19(12):2902-2905.

    [17].马慧敏,张文兵*,施小六,于志强,胡俊杰,宋任芳,盛国英,傅家谟, “人体结肠癌组织样品中基因组DNA甲基化水平的HPLC-ESI-MS/MS检测,分析测试学报, 2009, 5:292-296.

    [18]. Philipp Rathert, Arunkumar Dhayalan, Huimin Ma and Albert Jeltsch* “Specificity of protein lysine methyltransferases and methods for detection of lysine methylation of non-histone proteins” Moecular. BioSystems, 200841186 – 1190

    [19].Renfang Song Wenbing Zhang Huayong Chen Huimin Ma Yulian Dong Guoying Sheng Zhen Zhou and Jiamo Fu*“Characterization of G-rich and T-rich oligonucleotides using ion-pair reversed-phase high-performance liquid chromatography/tandem electrospray ionization mass spectrometry” European Journal of Mass Spectrometry, 200410705-713

    [20].Renfang Song Wenbing Zhang Huayong ChenHuimin MaYulian Dong Guoying Sheng Zhen Zhou and Jiamo Fu*, “Comparison of A+T rich oligonucleotides with and without self-complementary sequence using ion-pair reversed-phase high-performance liquid chromatography/tandem electrospray ionization mass spectrometry” European Journal of Mass Spectrometry2005, 1183-92

    [21].Huayong Chen Wenbing ZhangRenfang SongHuimin MaYulian Dong Guoying Sheng Zhen Zhou and Jiamo Fu*, “Analysis of DNA methylation by ion-pair reversed-phase high-performance liquid chromatography/tandem electrospray ionization mass spectrometry” Rapid Communications in Mass Spectrometry , 2004, 182773-2778

    [22].Renfang Song Wenbing Zhang Huayong ChenHuimin MaYulian Dong Guoying Sheng Zhen Zhou and Jiamo Fu*, “Site determination of phenyl glycidyl ether-DNA adducts using high-performance liquid chromatography tandem electrospray ionization mass spectrometry” Rapid Communications in Mass Spectrometry , 2005, 19: 1120-1124

    [23].Renfang Song Wenbing ZhangHuayong ChenHuimin MaYulian Dong Guoying Sheng Zhen Zhou and Jiamo Fu*“Analysis of SNPs sites in exon 4 of the P53 gene using high-performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry”, European Journal of Mass Spectrometry , 2006, 12: 205-211

    [24].Renfang Song Wenbing Zhang Huayong ChenHuimin MaYulian Dong, Guoying Sheng Zhen Zhou and Jiamo Fu*“Characterization of activation energy distribution for UV irradiation of T-rich oligonucleotide using high-performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry”, European Journal of Mass Spectrometry, 2006, 12 :283-289

    承担科研项目情况:
  • [1].国家自然科学面上项目,项目名称:大气细颗粒物中类腐殖酸类化合物的有效毒性组分的筛选和鉴定20211月到202412月,经费57万,项目号:42077197,课题负责人;

    [2].国家重点研发计划国合专项,2018YFE0106900,交通源超细颗粒物暴露与神经退行性疾病风险评估的关键技术及应用,2018YFE0106900 2019-72022-6570万,第三课题负责人;

    [3].广东省自然科学基金,面上项目,2019A1515011859 血铅诱导儿童智力降低的DNA甲基化生物标志物的初步鉴定,2019-102022-9 10万,主持人;

    [4]. 广州市科技计划项目,项目名称:广州市PM2.5中不同来源的类腐殖酸类化合物对人体肺细胞的毒性贡献20213-20233月,经费:5万,主持人。

    [5].国家基金重点项目,项目名称 “大气颗粒物中多环芳香有机质(POM)化学组成与毒性的分布和演化 20211月到202512月, 经费:304万,项目号:42030715,主要参与人员;

    [6].广州市科技计划科学研究专项重点项,201707020033,广州不同典型区域大气颗粒物中的毒害有机物及毒性效应,2017-52020-12, 200万元,主要参与人员;

    [7].国家自然科学青年基金,21007072 HPLC-MS/MS内标法研究典型污染物对人体DNA甲基化的影响,2011-12013-1219 万元,主持;

    [8].中国科学院知识创新工程重要方向项目, KZCX2-YW-GJ02, 南亚POPs污染及其跨境迁移趋向, 2010-12012-1, 190万元,第二课题主持人;

    [9].中国科学院广州地球化学研究所知识创新领域前沿项目,GIGCX-09-01,苯及其代谢物对人体肝脏和骨髓细胞基因的甲基化影响, 2009-112011-11 20万元,主持人。